Platelet Rich Plasma

Procedure for Preparing Platelet Rich Plasma

1. Draw the appropriate amount of anticoagulant
2. Transfer the anticoagulant into the plasma chamber (white part)
   1. 1mL for 20mL and 2 mL for 60mL
3. Draw blood from the patient (20 mL or 60 mL)
4. After drawing the blood, invert the syringe several times to ensure adequate mixing of the blood and anticoagulant.
5. Attach a blunt needle to the blood filled syringe and dispense contents into the blood chamber (red part)
6. Load the specimen in the centrifuge. Use appropriate balance weight (20 mL = 20 weight).
7. Close lid on machine and press green “Start” buttom. total processing time is 14 minutes.
8. Remove specimen when cycle is complete.
9. Using the plasma syringe with blunt cannula and yellow spacers, withdraw plasma volume from the white part to obtain
   1. 10 cc of PRP (do not remove any yellow spacers)
   2. 7 cc of PRP (remove 1/2 ince of the yellow spacers)
   3. 3 cc of PRP from 20 cc (do not remove any yellow spacers)
10. transfer the metal blunt needle from 20 mL syringe to 10 mL syringe and withdraw the PRP. Discard bubbles from the syringe.
11. Draw thrombin using 1 cc syringe from the vial (1 cc thrombin = 10 mL PRP)
12. Attach the syringe to the liquid applicators (gun)
13. Place bones in the cup and squirt the PRP/thrombin into the bone, just enough for the bones to be wet